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A donor splice site mutation c. The X specific Dmrt1 is truncated, while the Y specific Dmrt1 is normal and it's the candidate sex determination gene.

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The Scatophagus argus is a cultured marine teleost fish in which females grow faster and larger than males, therefore cloning the master sex-determination SD gene will be helpful for its sex control in aquaculture. A well-conserved Dmrt1 doublesex- and mabrelated transcription factor-1 gene, encoding zinc finger-like DNA binding motif DM-domain plays a critical role in sex determination and differentiation in vertebrates.

Both Markers were confirmed in adult S. These indicate that the sexes of S. A truncated Dmrt1 Dmrt1b was found to exist in both males and females.

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The Dmrt1b is with high similarity with Dmrt1 indicating Dmrt1b is the allele of Dmrt1 located on the X chromosome. RT-PCR revealed that Dmrt1 is highly expressed in the testes, while it is not expressed in the ovaries. View PDF. Access through your institution Purchase PDF. Aquaculture Volumemale scat Octobers Add to Mendeley Share. Abstract The Scatophagus argus is a cultured marine teleost fish in which females grow faster and larger than males, therefore cloning the master sex-determination SD gene will be helpful for its sex control in aquaculture.

Keywords Sex determination gene. SD sex-determination. Dmy DM-domain gene on the Y chromosome. ORF open reading frame. RT-PCR reverse transcript polymerase chain reaction.

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SRY sex-determining region on the Y chromosome. Single-nucleotide polymorphisms SNP. Recommended articles Citing articles 0. All rights reserved.

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